February 1st to 5th 2016
Olhão, Portugal
Tweet #MaresConference2016

Fast and cheap environmental DNA method to detect the invasive black-pygmy mussel Xenostrobus securis in water samples.

Oral Presentation
Biological Invasions
Thursday, February 4, 2016 -
17:00 to 17:15

Miralles, L. 1 Devloo-Delva, F. 2 Tsartsianidou, V. 3 García-Vázquez, E. 4 Ardura, A. 5

1University of Oviedo
2University of Oviedo
4University of Oviedo
5University of Oviedo

The black-pygmy mussel Xenostrobus securis is an invasive species that produces serious ecological and economical loss worldwide. It can occupy different niches and can adhere to different substrates. This invasive mussel can spread fast due to a wide physiological tolerance, together with a short generation time and high plasticity. Furthermore, Xenostrobus securis can carry the parasite Marteilia refringens that causes high mortality in local and commercial species. For all these reasons, its early-detection is essential for management purposes. In this work, a species-specific marker in the cytochrome c oxidase I (COI) gene were developed and tested in water samples from laboratory experiments (in-vitro conditions) as well as in nature experiments (Port of Avilés, North Spain). PCR protocol spent less than 2 hours and products are visualized in agarose gel, meaning a fast and cheap detection method. Positive amplification from water samples correlated with the physical presence of Xenostrobus securis confirming the value of the marker for environmental monitoring with good detection sensitivity.

Log in to upload your presentation