February 1st to 5th 2016
Olhão, Portugal
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A robust DNA-based marker for detection of invasive anadromous Salmonids from estuary water eDNA

Oral Presentation
Biological Invasions
Thursday, February 4, 2016 -
15:00 to 15:15

Clusa, L. 1 Ardura, A. 2 García-Vázquez, E. 3

1University of Oviedo
2University of Oviedo
3University of Oviedo

Many environmental changes alter today biological communities, for that reason the advent of genomic tools is becoming necessary to control invasive species. In the case of anadromous salmonids, they are widely spread over the world and have been introduced for aquaculture and sport fishing in many temperate regions. In their marine phase they use estuaries as gates for invading new river basins. Five species are in the list of the Global Invasive Species Database. Here we have developed a method to detect the presence of salmonids directly from water samples, focusing on estuaries of the Iberian Peninsula as a case study. We have designed Salmonidae-specific primers. From these primers, with a simple PCR-RFLP procedure the species present in the Peninsula can be unequivocally identified, like the native Atlantic salmon (Salmo salar) and Brown trout (Salmo trutta), and the invasive Rainbow trout (Oncorhynchus mykiss). The method was validated experimentally as well as from water samples with known Salmonid populations. This powerful and economical tool can be applied in routine monitoring of the presence of invasive anadromous salmonids on estuary waters.
Invasive, Salmonids, detection

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