February 1st to 5th 2016
Olhão, Portugal
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Effects of ocean acidification on biochemical biomarkers and swimming ability of the sand-smelt larvae

Oral Presentation
Future Oceans
Wednesday, February 3, 2016 -
10:15 to 10:30

Silva, C.S.E. 1 Novais, S.C. 2 Mendes, S. 3 Olveira, A.P. 4 Gonçalves, E.J. 5 Lemos, M.F.L. 6 Faria, A. 7

11. MARE – Marine and Environmental Sciences Centre 2. ESTM, Instituto Politécnico de Leiria, Portugal
21. MARE – Marine and Environmental Sciences Centre 2. ESTM, Instituto Politécnico de Leiria, Portugal
31. MARE – Marine and Environmental Sciences Centre 2. ESTM, Instituto Politécnico de Leiria, Portugal
4IPMA – Instituto Português do Mar e da Atmosfera, Algés, Portugal
5MARE – Marine and Environmental Science Centre, ISPA?IU, Lisboa, Portugal
6MARE –Marine and Environmental Sciences Centre, ESTM, Instituto Politécnico de Leiria, Portugal
7MARE – Marine and Environmental Science Centre, ISPA?IU, Lisboa, Portugal

The ocean acidification is a critical global problem and scientific investigation of itseffects is still in its infancy. Most research has been conducted on calcifying organisms andlittle attention has been given to the potential impact on other ecosystem processes andcomponents, like the early life-stages of non-calcifying organisms such as fish. In thiscontext, the main goal of this study was to investigate the effects of exposure to elevatedpCO2 on behavior, development, oxidative stress and metabolism of the early stages of sandsmelt,Atherina presbyter. Wild larvae in flexion and post-flexion stages were caught atArrábida Marine Park, Portugal and kept in controlled conditions with different pCO2 levels(Control: ~600?atm; Medium: ~1000?atm; High: ~1800?atm) between 7-15 days, beforebeing tested to assess critical swimming speed (Ucrit). Additionally, morphometricmeasurements and biochemical biomarkers were determined. The measured biomarkers wererelated with oxidative stress (SOD, CAT, LPO, DNA damage and ROS) and energymetabolism (total carbohydrate levels, ETS, LDH and IDH). Results suggest that swimmingbehavior of sand-smelt is unaffected by exposure to high pCO2 levels; however thebiochemical results suggest higher energetic costs may be associated with morphometricchanges and also with the exposure to a stressful environment. This study contributes withinformation on this species sensitivity to future ocean acidification conditions, showing thatdespite the absence of swimming behavior effects, the metabolic responses demonstrate anevidence of oxidative stress at elevated pCO2, whose future consequences are still unknown.
keywords: 
Acidification; fish; Ucrit; Oxidative stress; Sand-smelt

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